Bacterial communities associated with the midgut microbiota of wild Anopheles gambiae complex in Burkina Faso
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Date
2019-10
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Nature
Abstract
Plasmodium falciparum is transmitted by mosquitoes from the Anopheles gambiae sensu lato (s.l) species complex and is
responsible for severe forms of malaria. The composition of the mosquitoes’ microbiota plays a role in P. falciparum trans mission, so we studied midgut bacterial communities of An. gambiae s.l from Burkina Faso. DNA was extracted from 17
pools of midgut of mosquitoes from the Anopheles gambiae complex from six localities in three climatic areas, including
cotton-growing and cotton-free localities to include potential diferences in insecticide selection pressure. The v3–v4 region
of the 16S rRNA gene was targeted and sequenced using Illumina Miseq (2×250 nt). Diversity analysis was performed
using QIIME and R software programs. The major bacterial phylum was Proteobacteria (97.2%) in all samples. The most
abundant genera were Enterobacter (32.8%) and Aeromonas (29.8%), followed by Pseudomonas (11.8%), Acinetobacter
(5.9%) and Thorsellia (2.2%). No statistical diference in operational taxonomic units (OTUs) was found (Kruskal–Wallis
FDR—p>0.05) among the diferent areas, felds or localities. Richness and diversity indexes (observed OTUs, Chao1,
Simpson and Shannon indexes) showed signifcant diferences in the cotton-growing felds and in the agroclimatic zones,
mainly in the Sudano-Sahelian area. OTUs from seven bacterial species that mediate refractoriness to Plasmodium infec tion in An. gambiae s.l were detected. The beta diversity analysis did not show any signifcant diference. Therefore, a same
control strategy of using bacterial species refractoriness to Plasmodium to target mosquito midgut bacterial community and
afect their ftness in malaria transmission may be valuable tool for future malaria control eforts in Burkina Faso.
Description
This study explored the midgut bacterial community of An.
gambiae s.l, a malaria vector from Burkina Faso. No statis tical diference in OTU abundances was found among the
diferent areas, felds or localities. Therefore, the same con trol strategy to target mosquito midgut bacterial community
and afect their ftness by bacterial refractoriness species
to Plasmodium may be a valuable way for future malaria
control efforts in Burkina Faso. Richness and diversity
indexes showed signifcant diferences in the cotton-growing
felds and in the agroclimatic zones, mainly in the Sudano Sahelian area. Potential bacterial species refractorines to Plasmodium were detected, with a particular great abun dance in the Sahelian area where low rate of malaria has
been recorded
Keywords
Midgut bacteria · Anopheles gambiae s.l complex · Microbiota · Malaria
Citation
Félix Yameogo,Abdoul Razack Sare,Abdou Azaque Zoure,Bacterial communities associated with the midgut microbiota of wild Anopheles gambiae complex in Burkina Faso,Sprinf Nature,October 2919,Ghana