Cryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signalling

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dc.contributor.authorDomfeh, Seth A.
dc.contributor.authorNarkwa, Patrick W.
dc.contributor.authorQuaye, Osbourne
dc.date.accessioned2023-04-22T14:13:49Z
dc.date.available2023-04-22T14:13:49Z
dc.date.issued2021-06-02
dc.description.abstractBackground: Diverse signalling pathways are involved in carcinogenesis and one of such pathways implicated in many cancers is the interleukin 6/signal transducer and activator of transcription 3 (IL-6/STAT3) signalling pathway. Therefore, inhibition of this pathway is targeted as an anti-cancer intervention. This study aimed to establish the effect of cryptolepine, which is the main bioactive alkaloid in the medicinal plant Cryptolepis sanguinolenta, on the IL-6/STAT3 signalling pathway. Methods: First, the effect of cryptolepine on the IL-6/STAT3 pathway in human hepatoma cells (HepG2 cells) was screened using the Cignal Finder Multi-Pathway Reporter Array. Next, to confirm the effect of cryptolepine on the IL-6/STAT3 signalling pathway, the pathway was activated using 200 ng/mL IL-6 in the presence of 0.5–2 μM cryptolepine. The levels of total STAT3, p-STAT3 and IL-23 were assessed by ELISA. Results: Cryptolepine downregulated 12 signalling pathways including the IL-6/STAT3 signalling pathway and upregulated 17 signalling pathways. Cryptolepine, in the presence of IL-6, decreased the levels of p-STAT3 and IL-23 in a dose-dependent fashion. Conclusion: Our results demonstrated that cryptolepine inhibits the IL-6/STAT3 signalling pathway, and therefore cryptolepine-based remedies such as Cryptolepis sanguinolenta could potentially be used as an effective immunotherapeutic agent for hepatocellular carcinoma and other cancers.en_US
dc.description.sponsorshipACE: Cell Biology of Infectious and Non-Communicable Diseasesen_US
dc.identifier.citationdoi: 10.1186/s12906-021-03326-xen_US
dc.identifier.issn 2662-7671
dc.identifier.urihttp://hdl.handle.net/123456789/1514
dc.language.isoenen_US
dc.relation.ispartofseriesBMC Complementary Medicine and Therapies 21;Article number: 161 (2021)
dc.subjectCryptolepineen_US
dc.subjectIL-6/STAT3en_US
dc.subjectCarcinogenesisen_US
dc.subjectHepatomaen_US
dc.subjectAnti-canceren_US
dc.subjectKwadwo A. Kusien_US
dc.subjectGordon A. Awandareen_US
dc.subjectCharles Ansahen_US
dc.subjectAlimatu Salamen_US
dc.subjectMohamed Mutocheluhen_US
dc.titleCryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signallingen_US
dc.typeArticleen_US
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