Alteration of neutral-alpha glucosidase in seminal plasma and correlation with sperm motility among men investigated for infertility Nigeria: a cross-sectional study

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dc.contributor.authorMoronkeji, Muyiwa Adeleye
dc.contributor.authorEmokpae, Mathias Abiodun
dc.date.accessioned2023-05-21T21:08:12Z
dc.date.available2023-05-21T21:08:12Z
dc.date.issued2020-06-30
dc.description.abstractIntroduction: Proteomic studies are becoming popular lately among reproductive biologists in the diagnosis and management of several diseases including male infertility. Objective: To evaluate the level of Neutral-alpha Glucosidase (NAG) in the seminal plasma of men being investigated for infertility in and to correlate its activity with sperm motility. Materials and Methods: Four hundred men age range 23–60 years were consecutively recruited in the study. After physical and clinical evaluation, the semen was collected by self or assisted masturbation and analysis was done according to the World Health Organization guidelines. The semen was then centrifuged and seminal plasma separated for the assay of NAG activity by ELISA technique using reagents supplied by Melson Medical Co, Shangai, China within one week of collection. The participants were categorized in to normozoospermia (191), oligozoospermia (98), severe oligozoospermia (70) and azoospermia (41) based on sperm count. Results: The mean levels of NAG activity decreased with decreasing levels of sperm count with values lowest among azoospermia and highest among normozoospermic subjects. The comparison of NAG activity between the various categories and control subjects was significantly different (P<0.001) except for normozoospermia which was not significantly different (P>0.05) when compared with controls. The mean NAG activity level was significantly lower among the asthenozoospermia (P<0.001) compared with normozoospermia. The NAG activity correlated positively with percentage sperm motility (r=0.126; P<0.02) and percentage sperm count (r=0.107; P<0.05). Conclusion: The findings indicate that NAG can be used for the assessment of semen quality as an adjunct to traditional semen analysis.en_US
dc.description.sponsorshipACE: Reproductive Health Innovationen_US
dc.identifier.citation: Moronkeji MA, Emokpae MA. Alteration of neutral alpha glucosidase in seminal plasma and correlation with sperm motility among men investigated foren_US
dc.identifier.issn2394-4285
dc.identifier.issn2468-919X
dc.identifier.urihttp://hdl.handle.net/123456789/1867
dc.language.isoenen_US
dc.publisherFertility Science and Researchen_US
dc.relation.ispartofseriesFertility Science and Research;2020:7
dc.subjectAstenozoospermiaen_US
dc.subjectmale infertilityen_US
dc.subjectneutral α-glucosidaseen_US
dc.subjectsemen analysisen_US
dc.subjectACE: Reproductive Health Innovationen_US
dc.subjectCERHIen_US
dc.subjectUniversity of Beninen_US
dc.subjectNigeriaen_US
dc.titleAlteration of neutral-alpha glucosidase in seminal plasma and correlation with sperm motility among men investigated for infertility Nigeria: a cross-sectional studyen_US
dc.typeArticleen_US
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