Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species

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dc.contributor.authorDingwoke, Emeka John
dc.contributor.authorAdamude, Fatima Amin
dc.contributor.authorMohamed, Gadija
dc.date.accessioned2023-05-04T17:03:15Z
dc.date.available2023-05-04T17:03:15Z
dc.date.issued2021-11-02
dc.description.abstractnakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophilic interaction liquid chromatography coupled with LC-MS/MS was used to analyze the crude venoms of Echis ocellatus (Carpet viper) and Bitis arietans (Puff adder) in order to understand their venom proteomic identities. Results obtained revealed that gel-free proteomic analysis of the crude venoms led to the identification of 85 and 79 proteins, respectively. Seventy-eight (78) proteins were common between the two snake species with a 91.8% similarity score. The identified proteins belong to 18 protein families in E. ocellatus and 14 protein families in B. arietans. Serine proteases (22.31%) and metalloproteinases (21.06%) were the dominant proteins in the venom of B. arietans; while metalloproteinases (34.84%), phospholipase A2s (21.19%) and serine proteases (15.50%) represent the major toxins in the E. ocellatus venom. Other protein families such as three-finger toxins and cysteine-rich venom proteins were detected in low proportions. This study provides an insight into the venom proteomic analysis of the two Nigerian viper species, which could be useful in identifying the toxin families to be neutralized in case of envenomationen_US
dc.description.sponsorshipACE: Neglected Tropical Diseases and Forensic Biotechnologyen_US
dc.identifier.citationhttps://doi.org/10.1016/j.bbrep.2021.101164en_US
dc.identifier.issn2405-5808
dc.identifier.urihttp://hdl.handle.net/123456789/1704
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofseriesBiochemistry and Biophysics Reports;28 (2021)
dc.subjectNeglected tropical diseaseen_US
dc.subjectSnakebiteen_US
dc.subjectVenom-based antivenomen_US
dc.subjectViperidaeen_US
dc.subjectBitis arietansen_US
dc.subjectEchis ocellatusen_US
dc.subjectSnake venomen_US
dc.subjectProteomicen_US
dc.subjectMass spectrometryen_US
dc.subjectAhmadu Bello Universityen_US
dc.subjectAshwil Kleinen_US
dc.subjectAliyu Salihuen_US
dc.subjectMujitaba Suleiman Abubakaren_US
dc.titleVenom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake speciesen_US
dc.typeArticleen_US
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