Comparison of leucocyte profiles between healthy children and those with asymptomatic and symptomatic Plasmodium falciparum infections

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dc.contributor.authorPrah, Diana Ahu
dc.contributor.authorAmoah, Linda Eva
dc.contributor.author. Gibbins, Matthew P
dc.date.accessioned2023-04-27T15:08:18Z
dc.date.available2023-04-27T15:08:18Z
dc.date.issued2020-10-09
dc.description.abstractBackground: The immune mechanisms that determine whether a Plasmodium falciparum infection would be symptomatic or asymptomatic are not fully understood. Several studies have been carried out to characterize the associations between disease outcomes and leucocyte numbers. However, the majority of these studies have been conducted in adults with acute uncomplicated malaria, despite children being the most vulnerable group. Methods: Peripheral blood leucocyte subpopulations were characterized in children with acute uncomplicated (symptomatic; n=25) or asymptomatic (n=67) P. falciparum malaria, as well as malaria-free (uninfected) children (n=16) from Obom, a sub-district of Accra, Ghana. Leucocyte subpopulations were enumerated by flow cytometry and correlated with two measures of parasite load: (a) plasma levels of P. falciparum histidine-rich protein 2 (PfHRP2) as a proxy for parasite biomass and (b) peripheral blood parasite densities determined by microscopy. Results: In children with symptomatic P. falciparum infections, the proportions and absolute cell counts of total (CD3+) T cells, CD4+T cells, CD8+T cells, CD19+B cells and CD11c+dendritic cells (DCs) were significantly lower as compared to asymptomatic P. falciparum-infected and uninfected children. Notably, CD15+neutrophil proportions and cell counts were significantly increased in symptomatic children. There was no significant difference in the proportions and absolute counts of CD14+monocytes amongst the three study groups. As expected, measures of parasite load were significantly higher in symptomatic cases. Remarkably, PfHRP2 levels and parasite densities negatively correlated with both the proportions and absolute numbers of peripheral leucocyte subsets: CD3+T, CD4+T, CD8+T, CD19+B, CD56+NK, γδ+T and CD11c+cells. In contrast, both PfHRP2 levels and parasite densities positively correlated with the proportions and absolute numbers of CD15+cells. Conclusions: Symptomatic P. falciparum infection is correlated with an increase in the levels of peripheral blood neutrophils, indicating a role for this cell type in disease pathogenesis. Parasite load is a key determinant of peripheral cell numbers during malaria infectionsen_US
dc.description.sponsorshipACE: Cell Biology of Infectious and Non-Communicable Diseasesen_US
dc.identifier.citationrah, D.A., Amoah, L.E., Gibbins, M.P. et al. Comparison of leucocyte profiles between healthy children and those with asymptomatic and symptomatic Plasmodium falciparum infections. Malar J 19, 364 (2020). https://doi.org/10.1186/s12936-020-03435-xen_US
dc.identifier.issn1475-2875
dc.identifier.urihttp://hdl.handle.net/123456789/1617
dc.language.isoenen_US
dc.publisherMalaria Journalen_US
dc.relation.ispartofseriesMalaria Journal;19, 364
dc.subjectYaw Bediakoen_US
dc.subjectAubrey J. Cunningtonen_US
dc.subjectUniversity of Ghanaen_US
dc.subjectWACCBIP_NCDSen_US
dc.subjectPathogenesisen_US
dc.subjectImmunityen_US
dc.subjectNeutrophilsen_US
dc.subjectLeucocytesen_US
dc.subjectAsymptomaticen_US
dc.subjectSymptomaticen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectmalariaen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectJulius Clemence R. Hafallaen_US
dc.subjectACE: Cell Biology of Infectious and Non-Communicable Diseasesen_US
dc.titleComparison of leucocyte profiles between healthy children and those with asymptomatic and symptomatic Plasmodium falciparum infectionsen_US
dc.typeArticleen_US
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