Antimicrobial and Toxicity Studies on Holisa Herbal Formulation
Loading...
Date
2021-06
Journal Title
Journal ISSN
Volume Title
Publisher
Tropical Journal of Natural Product Research
Abstract
Herbal plants have been reported to play crucial role in enhancing human health. The study
evaluated the antimicrobial property, acute and sub-chronic toxicological effects of Holisa
herbal formulation consisting of Vernonia amygdalina, Rorippa madgasceriensis, and
Securinega virosa plants using biochemical and histopathological indices. Thirty (30) male
Wistar rats of five groups (A-E) of 6 animals each were used. Animals in group A were given
distilled water while those in B, C, D, and E received 20, 40, 60, 100 mL/kg of body weight of
the formulation for 28 days. The zone of inhibition was determined with agar well diffusion
method using seven bacterial and three fungal isolates. The acute toxicity was carried out with
the use of Swiss mice which were observed for 72 hours. The zone of inhibition observed on
both the bacterial and fungal culture media was very minimal (6-9 mm). Significant (p<0.05)
increase in body weight was observed in groups B, C, and E and a significant (p<0.05) decrease
in group D when compared to the control. Alkaline phosphatase, direct bilirubin, triglyceride,
total cholesterol, low-density lipoprotein, high-density lipoprotein, creatinine, and total protein
were increased significantly (p<0.05), while aspartate aminotransferases, alanine
aminotransferases, albumin did not show significant differences. The histopathology conducted
showed that the formulation showed congestion in the blood vessels and vascular constriction on
the liver and kidney tissues respectively. The results revealed that lower doses of Holisa herbal
formulation may not have any adverse effect on the liver and kidney tissues but high doses
elicited toxicity
Description
Keywords
Holisa herbal formulation, Antimicrobial studies, Biochemical studies, Histopathology, Nigeria, Digital Development, Covenant University, ACE: Applied Informatics and Communication