Antisickling effect of chrysin is associated with modulation of oxygenated and deoxygenated haemoglobin via alteration of functional chemistry and metabolic pathways of human sickle erythrocytes

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dc.contributor.authorNwankwo, H.C.
dc.contributor.authorIdowu, A.A.
dc.contributor.authorMuhammad, A.
dc.date.accessioned2023-04-28T10:00:28Z
dc.date.available2023-04-28T10:00:28Z
dc.date.issued2021-06-21
dc.description.abstractSickle cell disease (SCD) treatment and management remain a challenging puzzle especially among developing Nations. Chrysin’s sickling-suppressive properties in human sickle (SS) erythrocytes in addition to its effect on AA-genotype erythrocytes were evaluated. Sickling was induced (76%) with 2% sodium metabisulphite at 3 h. Chrysin prevented (81.19%) the sickling and reversed same (84.63%) with strong IC50s (0.0257 mM and 0.00275 mM, respectively). The levels of oxygenated haemoglobin in the two groups (before and after induction approaches) were similar but significantly (P < 0.05) higher than that of SS erythrocytes (the ‘induced’ control), with chrysin-treated AA-genotype showing no effects relative to the untreated. The level of deoxygenated haemoglobin in the ‘induced’ control group was significantly (P < 0.05) higher than those of the chrysin-treated SS erythrocytes. Normal and chrysin-untreated erythrocytes (AA-untreated) were significantly more resistant to osmotic fragility than the SS-untreated. However, treatment with chrysin significantly reduced the osmotic fragility of the cells relative to the untreated cells. Furthermore, chrysin treatment significantly lowers the high level of 2,3-diphosphoglycerate (2,3-DPG) observed in the sickle erythrocytes, with no effects on AA-genotype erythrocytes. Based on functional chemistry, chrysin treatment alters the functional groups in favour of its antisickling effects judging from the observed bends and shifts. From metabolomics analysis, it was observed that chrysin treatment favors fatty acid alkyl monoesters (FAMEs) production with concomitant shutting down-effects on selenocompound metabolism. Thus, sickling-suppressive effects of chrysin could potentially be associated with modulation of oxygenated and deoxygenated haemoglobin via alteration of human sickle erythrocyte’s functional chemistry and metabolic pathways implicated in SCD crisisen_US
dc.description.sponsorshipACE: Neglected Tropical Diseases and Forensic Biotechnologyen_US
dc.identifier.citationwankwo HC, Idowu AA, Muhammad A, Waziri AD, Abubakar YS, Bashir M, Erukainure OL. Antisickling effect of chrysin is associated with modulation of oxygenated and deoxygenated haemoglobin via alteration of functional chemistry and metabolic pathways of human sickle erythrocytes. Hum Exp Toxicol. 2021 Dec;40(12_suppl):S108-S124. doi: 10.1177/09603271211025599. Epub 2021 Jun 21. PMID: 34151613.en_US
dc.identifier.issn0960-3271
dc.identifier.urihttp://hdl.handle.net/123456789/1624
dc.language.isoenen_US
dc.publisherSageen_US
dc.relation.ispartofseriesHuman and Experimental Toxicology;2021, Vol. 40(12
dc.subjectSicklingen_US
dc.subjectchrysinen_US
dc.subjecteffectsen_US
dc.subjecthaemoglobinen_US
dc.subjectmodulationen_US
dc.subjectmetabolomicsen_US
dc.subjectA.D. Waziren_US
dc.subjectY.S. Abubakaren_US
dc.subjectAhmadu Bello Universityen_US
dc.subjectACENTDFBen_US
dc.subjectACE: Neglected Tropical Diseases and Forensic Biotechnologyen_US
dc.titleAntisickling effect of chrysin is associated with modulation of oxygenated and deoxygenated haemoglobin via alteration of functional chemistry and metabolic pathways of human sickle erythrocytesen_US
dc.typeArticleen_US
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