Helicobacter Pylori Variants with ABC-Type Tyrosine Phosphorylation Motif in Gastric Biopsies of Ghanaian Patients

Simple item page
dc.contributor.authorTagoe, Emmanuel A
dc.contributor.authorAwandare, Gordon A.
dc.contributor.authorQuaye, Osbourne
dc.date.accessioned2023-04-24T12:59:57Z
dc.date.available2023-04-24T12:59:57Z
dc.date.issued2021-03-31
dc.description.abstractBackground. Helicobacter pylori pathogenicity and disease severity are determined by the tyrosine phosphorylation motifs of CagA protein. This study is aimed at detecting the presence of H. pylori and identifying the CagA tyrosine phosphorylation motifs in Ghanaian patients. Material and Methods. A total of 94 archival genomic DNA samples from gastric biopsies were used for the study, and H. pylori was detected by amplifying the 16S rRNA gene. The 3′-end variable region of the cagA gene was amplified,and the entire 3′-end was sequenced and translated into amino acids. Results. H. pylori was detected in 53.2% (50/94) of the samples, and all the detected bacteria harboured the cagA gene. Two variants of the bacteria were identified based on the size of the amplified cagA gene: 207 bp and 285 bp. The 207 bp and 285 bp variants accounted for 74% and 22%, respectively, and 4% showed both fragments. Translated amino acid sequence of the cagA gene showed EPIYA-A, EPIYA-B, and EPIYA-C (ABC type) motifs, indicating the Western variant. The CagA protein C-terminal showed insertion of amino acids in the sequence flanking the EPIYA-A motif at the N-terminal and a complete deletion of the EPIYA-CC and EPIYA-CCC motifs together with the flanking sequences. Conclusions. H. pylori identified were Western variant (ABC type) with unique amino acid insertions, suggesting unique variants in Ghanaian patients. Further investigation is however required to understand the role of the molecular diversity of the variant in gastric disease outcomeen_US
dc.description.sponsorshipACE: Cell Biology of Infectious and Non-Communicable Diseasesen_US
dc.identifier.citationTagoe, E. A., Awandare, G. A., Quaye, O., Asmah, R. H., Archampong, T. N., Osman, M. A., & Brown, C. A. (2021). Helicobacter pylori Variants with ABC-Type Tyrosine Phosphorylation Motif in Gastric Biopsies of Ghanaian Patients. BioMed Research International, 2021.en_US
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.urihttp://hdl.handle.net/123456789/1562
dc.language.isoenen_US
dc.publisherHindawien_US
dc.relation.ispartofseriesBiomed Res Int;2021
dc.subjectRichard H. Asmahen_US
dc.subjectTimothy N. Archampongen_US
dc.subjectMahasin A. Osmanen_US
dc.subjectCharles A. Brownen_US
dc.subjectUniversity of Ghanaen_US
dc.subjectWACCBIP_NCDSen_US
dc.subjectgastric diseaseen_US
dc.subjecttyrosine phosphorylation motifsen_US
dc.subjectCagA proteinen_US
dc.titleHelicobacter Pylori Variants with ABC-Type Tyrosine Phosphorylation Motif in Gastric Biopsies of Ghanaian Patientsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Helicobacter Pylori Variants.pdf
Size:
1.09 MB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Health