Ameliorative Effect of Zingiber officinale on Chemical Induced DNA Damage in Rats Using PCR Analysis
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Date
2020-09-12
Journal Title
Journal ISSN
Volume Title
Publisher
Biointerface Research in Applied Chemistry
Abstract
Ginger rhizomes have been reportedly used in folk medicine for the management of various
ailments. This study, therefore, investigates the ameliorative effect of the ethanolic extract of ginger
(Zingiber officinale) rhizomes against DNA damage in rats induced with different carcinogens. Fifteen
Wistar rats grouped into 3 of 5 rats per group were used for the study. The first set of blood samples
was first collected before the animals were orally treated with heavy metals. After 14 days of induction,
the second set of blood was collected. The third phase of blood collection was done after administering
an ethanolic extract of Z. officinale for 14 days. The UV wavelength absorption spectrum and
conventional PCR analysis were carried out on DNA extracts of all the animals. Cluster analysis of
optical density (OD) and PCR data were carried out as well as genomic instability, similarity, and
diversity using the best 3 Random Amplified Polymorphic DNA (RAPD) primers. The PCR –DNA
concentration analysis showed the Z. officinale extract's ameliorative effect against lead acetate,
cadmium chloride, and arsenic trioxide-induced DNA damage with a significant (p < 0.05) reduction in
DNA concentration of the treated rats when compared with induced rats. The cluster analysis of optical
density values revealed close similarity between the control animals' DNA, a slight similarity with
treated animals' DNA, and a significant difference with the induced animal DNA. These results
indicated the ameliorative properties of Z. officinale against these heavy metals induced DNA damage
in rats
Description
Keywords
Zingiber officinale, polymerase chain reaction, RAPD- PCR Primer, heavy metals, Nigeria, Digital Development, Covenant University, CAPiC, ACE: Applied Informatics and Communication, Amos Onasanya