Molecular epidemiology of Mycobacterium tuberculosis complex in the Volta Region of Ghana
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Date
2021-03-17
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS ONE
Abstract
Context
Available molecular epidemiological data from recent studies suggest significant genetic
variation between the different lineages of Mycobacterium tuberculosis complex (MTBC)
and the MTBC lineages might have adapted to different human populations.
Aim
This study sought to determine the population structure of clinical MTBC isolates from the
Volta Region of Ghana.
Methods
The MTBC isolates obtained from collected sputum samples were identified by PCR detect ing of IS6110 and genotyped using spoligotyping. Non-tuberculous mycobacterial isolates
were characterized by amplification of the heat shock protein 65 (hsp65) gene and sequenc ing. The drug susceptibility profiles of the MTBCs determined using GenoType MTBDRplus.
Results One hundred and seventeen (117, 93.6%) out of 125 mycobacterial positive isolates were
characterized as members of the MTBC of which M. tuberculosis sensu stricto (MTBss) and
M. africanum (MAF) were respectively 94 (80.3%) and 23 (19.7%). In all, 39 distinct spoligo type patterns were obtained; 26 for MTBss and 13 for MAF lineages. Spoligotyping identified
89 (76%) Lineage 4, 16 (13.6%) Lineage 5, 7 (6.0%) Lineage 6, 3 (2.6%) Lineage 2, 1(0.9%)
Lineage 3 and 1 (0.9%) Lineage 1. Among the Lineage 4 isolates, 62/89 (69.7%) belonged
to Cameroon sub-lineage, 13 (14.7%) Ghana, 8 (9.0%) Haarlem, 2 (2.2%) LAM, 1 (1.1%)
Uganda I, 1 (1.1%) X and the remaining two (2.2%) were orphan. Significant localization of
MAF was found within the Ho municipality (n = 13, 29.5%) compared to the more cosmopoli tan Ketu-South/Aflao (n = 3, 8.3%) (p-value = 0.017). Eight (8) non-tuberculous mycobacte ria were characterized as M. abscessus (7) and M. fortuitum (1 Conclusion We confirmed the importance of M. africanum lineages as a cause of TB in the Volta region of Ghana
Description
Keywords
Isaac Darko Otchere, University of Ghana, WACCBIP_NCDS, Stephen Osei-Wusu, Dorothy Yeboah-Manu, Adwoa Asante-PokuI
Citation
EN ACCESS Citation: Ameke S, Asare P, Aboagye SY, Otchere ID, Osei-Wusu S, Yeboah-Manu D, et al. (2021) Molecular epidemiology of Mycobacterium tuberculosis complex in the Volta Region of Ghana. PLoS ONE 16(3): e0238898.