Translational suppression of SARS-COV-2 ORF8 protein mRNA as a Viable therapeutic target against COVID-19: Computational studies on potential roles of isolated compounds from Clerodendrum volubile leaves

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dc.contributor.authorErukainure, Ochuko L.
dc.contributor.authorAtolani, Olubunmi
dc.contributor.authorMuhammad, Aliyu
dc.date.accessioned2023-05-04T17:03:39Z
dc.date.available2023-05-04T17:03:39Z
dc.date.issued2021-10-19
dc.description.abstractThe open reading frame 8 (ORF8) protein of SARS-CoV-2 has been implicated in the onset of cytokine storms, which are responsible for the pathophysiology of COVID-19 infection. The present study investigated the po tential of isolated compounds from Clerodendrum volubile leaves to stall oxidative bursts in vitro and interact with ORF8 mRNA segments of the SARS-CoV-2 whole genome using computational tools. Five compounds, namely, harpagide, 1-(3-methyl-2-butenoxy)-4-(1-propenyl)benzene, ajugoside, iridoid glycoside and erucic acid, were isolated from C. volubile leaves, and their structures were elucidated using conventional spectroscopy tools. Iridoid glycoside is being reported for the first time and is thus regarded as a new compound. The ORF8 mRNA sequences of the translation initiation sites (TIS) and translation termination sites (TTSs) encoding ORF8 amino acids were retrieved from the full genome of SARS-CoV-2. Molecular docking studies revealed strong molecular interactions of the isolated compounds with the TIS and TTS of ORF8 mRNA. Harpagide showed the strongest binding affinity for TIS, while erucic acid was the strongest for TTS. The immunomodulatory potentials of the isolated compounds were investigated on neutrophil phagocytic respiratory bursts using luminol-amplified chemiluminescence technique. The compounds significantly inhibited oxidative burst, with 1-(3-methyl-2- butenoxy)-4-(1-propenyl)benzene having the best activity. Ajugoside and erucic acid showed significant inhib itory activity on T-cell proliferation. These results indicate the potential of C. volubile compounds as immuno modulators and can be utilized to curb cytokine storms implicated in COVID-19 infection. These potentials are further corroborated by the strong interactions of the compounds with the TIS and TTS of ORF8 mRNA from the SARS-CoV-2 whole genome.en_US
dc.description.sponsorshipACE: Neglected Tropical Diseases and Forensic Biotechnologyen_US
dc.identifier.issn0010-4825
dc.identifier.urihttp://hdl.handle.net/123456789/1706
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesComputers in Biology and Medicine;139 (2021)
dc.subjectClerodendrum volubileen_US
dc.subjectCOVID-19en_US
dc.subjectCytokine stormen_US
dc.subjectIridoid glycosideen_US
dc.subjectORF8en_US
dc.subjectAnd SARS-CoV-2en_US
dc.subjectAhmadu Bello Universityen_US
dc.subjectACENTDFBen_US
dc.subjectACENTDFBen_US
dc.subjectRahul Ravichandranen_US
dc.subjectRahul Ravichandranen_US
dc.titleTranslational suppression of SARS-COV-2 ORF8 protein mRNA as a Viable therapeutic target against COVID-19: Computational studies on potential roles of isolated compounds from Clerodendrum volubile leavesen_US
dc.typeArticleen_US
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