Annona senegalensis extract demonstrates anticancer properties in N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar rats

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dc.contributor.authorYakubu, Omolara F.
dc.contributor.authorMetibemu, Damilohun S.
dc.contributor.author. Adelani, Isaacson B
dc.date.accessioned2023-08-29T15:26:49Z
dc.date.available2023-08-29T15:26:49Z
dc.date.issued2020
dc.description.abstractBackground: Hepatocellular carcinoma (HCC) is a common and leading cancer around the globe. This study investigated the anticancer properties of extract of Annona senegalensis in N-diethylnitrosamine (DEN) - induced hepatocellular carcinoma in male Wistar rats. Methods: Rats were simultaneously induced with a combination of 100 mg/kg b.wt of DEN and 0.5 mL/kg of carbon tetrachloride (CCl4) intraperitoneally once a week for three weeks in a row. Thereafter, animals were treated with 100 mg/kg and 200 mg/kg b.wt of A. senegalensis extract daily for 21days. Analysis using gas chromatography-mass spectrometry (GC–MS) was carried out to discover the phytoconstituents contained in the n-hexane extract of A. senegelensis. The levels of liver function parameters and antioxidant enzyme activities were determined via spectrophotometric analysis. Reverse transcriptase-polymerase chain reaction technique was used to assess the gene expression patterns of BCL-2, P53, P21, IL-6, FNTA, VEGF, HIF, AFP, XIAP, and EGFR mRNAs. Results: Treatment of DEN-induced hepatocellular carcinoma Wistar rats with the extract caused significant (p < 0.05) decrease in the activities of ALT and AST. It also resulted in a reduction of the concentration of MDA and a significant increase (p < 0.05) in SOD and GSH activities. IL-6, BCL-2, VEGF, EGFR, XIAP, FNTA, and P21 mRNAs expressions were significantly (p < 0.05) downregulated after treatment. Histopathological analysis revealed that the extract improved the liver architecture. Conclusion: A. senegelensis n-hexane extract demonstrates its anticancer properties by improving the liver architecture, increasing the antioxidant defense systems, downregulating the pro-inflammatory, anti-apoptotic, angiogenic, alpha-fetoprotein and farnesyl transferase mRNAs expression and hitherto up-regulate the expression of tumor suppressor (P21 and P53) mRNAs.en_US
dc.description.sponsorshipACE: Applied Informatics and Communicationen_US
dc.identifier.issn0753-3322
dc.identifier.urihttp://hdl.handle.net/123456789/2088
dc.language.isoenen_US
dc.publisherBiomedicine & Pharmacotherapyen_US
dc.relation.ispartofseriesBiomedicine & Pharmacotherapy;131 (2020)
dc.subjectAnnona senegalensisen_US
dc.subjectHepatocellular carcinomaen_US
dc.subjectN-Diethylnitrosamineen_US
dc.subjectGene expressionen_US
dc.subjectNigeriaen_US
dc.subjectDigital Developmenten_US
dc.subjectCovenant Universityen_US
dc.subjectCAPiCen_US
dc.subjectACE: Applied Informatics and Communicationen_US
dc.titleAnnona senegalensis extract demonstrates anticancer properties in N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar ratsen_US
dc.typeArticleen_US
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