Immune Response in Mice Immunized with Chimeric H1 Antigens

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dc.contributor.authorKotey, Erasmus Nikoi
dc.contributor.authorAmpofo, William Kwabena
dc.contributor.authorDaines, Rebecca
dc.date.accessioned2023-04-22T14:17:12Z
dc.date.available2023-04-22T14:17:12Z
dc.date.issued2021-10-15
dc.description.abstract: Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds potential for further development of a universal influenza vaccine.en_US
dc.description.sponsorshipACE: Cell Biology of Infectious and Non-Communicable Diseasesen_US
dc.identifier.citationhttps://doi.org/10.3390/vaccines9101182en_US
dc.identifier.issn2076-393X
dc.identifier.issn0000-0000
dc.identifier.urihttps://datad.aau.org/handle/123456789/1525
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofseriesVaccines;2021, 9, 1182
dc.subjectuniversal influenza vaccineen_US
dc.subjectbroad-reactive antibodiesen_US
dc.subjectchimeric haemagglutininen_US
dc.subjecttherapeutic; prophylacticen_US
dc.subjectseasonal influenzaen_US
dc.subjectcross-reactivityen_US
dc.subjectJean-Remy Sadeyenen_US
dc.subjectMunir Iqbalen_US
dc.subjectOsbourne Quayeen_US
dc.titleImmune Response in Mice Immunized with Chimeric H1 Antigensen_US
dc.typeArticleen_US
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