HIV-associated nephropathy: Protocol and rationale for an exploratory genotype phenotype study in a sub-Saharan African population

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dc.contributor.authorYusuf, Aminu Abba
dc.contributor.authorMusa, Baba Maiyaki
dc.contributor.authorGaladanci, Najibah Aliyu
dc.date.accessioned2023-06-07T17:37:55Z
dc.date.available2023-06-07T17:37:55Z
dc.date.issued2021-04-06
dc.description.abstractBackground HIV-positive persons of African descent are disproportionately affected by chronic kidney disease (CKD). Deterioration to end-stage kidney disease (ESKD) also occurs in this population at a higher frequency. There remains a lot to learn about the genetic susceptibility to CKD in HIV positive patients, and the pathophysiology of progression to ESKD. Objectives We will conduct an exploratory genotype-phenotype study in HIV-positive persons with CKD in Aminu Kano Teaching Hospital, Nigeria, to determine blood-based differential gene expression biomarkers in different kidney risk groups according to the KDIGO 2012 criteria. Methods We will consecutively screen 150 HIV-positive adults (�18 years of age) attending the HIV clinic of Aminu Kano Teaching Hospital, Kano, Nigeria, for CKD based on proteinuria and elevation of estimated glomerular filtration rate. Among these, two separate groups of 16 eligible participants each (n = 32) will be selected in the four (4) KDIGO 2012 kidney risk categories. The groups will be matched for age, sex, viral suppression level and antiretroviral (ARV) regimen. In the first group (n = 16), we will determine differential gene expression markers in peripheral blood mononuclear cells using mRNA-sequencing (RNA-Seq). We will validate the differential expression markers in the second group (n = 16) using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Using a systems-based approach, we will construct, visualize and analyze gene-gene interaction networks to determine the potential biological roles of identified differential expression markers based on published literature and publicly available databases. Results Our exploratory study will provide valuable information on the potential roles of differential expression biomarkers in the pathophysiology of HIV-associated kidney disease by identifying novel biomarkers in different risk categories of CKD in a sub-Saharan African population. The results of this study will provide the basis for population-based genome-wide association studies to guide future personalized medicine approaches. Conclusion Validated biomarkers can be potential targets for the development of stage-specific therapeutic interventions, an essential paradigm in precision medicine.en_US
dc.description.sponsorshipACE: Genetic Medicineen_US
dc.identifier.citationYusuf AA, Musa BM, Galadanci NA, Babashani M, Mohammed AZ, Ingles DJ, et al. (2021) HIV-associated nephropathy: Protocol and rationale for an exploratory genotype-phenotype study in a sub-Saharan African population. PLoS ONE 16(4): e0249567. https://doi.org/10.1371/ journal.pone.0249567en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/123456789/1920
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.relation.ispartofseriesPLoS ONE;16(4)
dc.subjectHIV-positive personsen_US
dc.subjectMusa Babashanien_US
dc.subjectAminu Zakari Mohammeden_US
dc.subjectDonna J. Inglesen_US
dc.subjectACE: Genetic Medicineen_US
dc.subjectUniversity of Ghanaen_US
dc.subjectWAGMCen_US
dc.titleHIV-associated nephropathy: Protocol and rationale for an exploratory genotype phenotype study in a sub-Saharan African populationen_US
dc.typeArticleen_US
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