Da, Dari F.McCabe, Ruth. Somé, Bernard M2023-06-092023-06-092021-04-212045-2322http://hdl.handle.net/123456789/1929There is an urgent need for high throughput, afordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected feld caught mosquitoes. Models trained on feld mosquitoes were unable to predict the infection status of other feld mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specifcity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.enPedro M. EsperançaKatarzynaA. SalaNigeriaACE: Mycotoxin and Food SafetyACEMFSFederal University of Technology, MinnaNigeriaJosua BlightDetection of Plasmodium falciparum in laboratory‑reared and naturally infected wild mosquitoes using near‑infrared spectroscopyArticle