Ikewuchi, Catherine C.. Ikewuchi, Jude CIfeanacho, Mercy O.2023-05-152023-05-152021Ikewuchi CC, Ikewuchi JC, Ifeanacho MO, Jack DP, Ikpe CN, Ehiosun S, Ajayi TB. Protective effect of aqueous leaf extracts of Chromolaena odorata and Tridax procumbens on doxorubicin-induced hepatotoxicity in Wistar rats. Porto Biomed J. 2021 Dec 3;6(6):e143. doi: 10.1097/j.pbj.0000000000000143. PMID: 34881354; PMCID: PMC8647896.2444-8664http://hdl.handle.net/123456789/1787Background: The liver is one of the organs affected by doxorubicin toxicity. Therefore, in this study, the potential protective role of aqueous leaf extracts of Chromolaena odorata and Tridax procumbens against doxorubicin-induced hepatotoxicity was investigated. Methods: In order to achieve this, their impact on hepatic biomarkers of oxidative stress, lipid and electrolytes’ profile, and plasmabiomarkers of liver functions/integrity were monitored in doxorubicin treated rats. The animals were treated with either metformin(250mg/kg body weight orally for 14 days) or the extracts (50, 75, and 100mg/kg orally for 14 days) and/or doxorubicin (15mg/kg,intraperitoneal, 48 h before sacrifice). Results: The hepatic malondialdehyde, cholesterol, calcium, and sodium concentrations, and plasma activities of alanine andaspartate transaminases and alkaline phosphatase, as well as plasma albumin to globulin ratio of test control were significantly(P<.05) higher than those of all the other groups. However, the plasma albumin, total protein, globulin, and total bilirubinconcentrations; hepatic concentrations of ascorbic acid, chloride, magnesium, and potassium; and hepatic activities of catalase,glutathione peroxidase, and superoxide dismutase of test control were significantly (P<.05) lower than those of all the other groups. Conclusions: Pretreatment with the extracts and metformin prevented to varying degrees, doxorubicin-induced hepatic damage,as indicated by the attenuation of doxorubicin-induced adverse alterations in hepatic biomarkers of oxidative stress, lipid andelectrolyte profiles, and plasma biomarkers of hepatic function/integrity, and keeping them at near-normal values.encholesterolChromolaena odoratadoxorubicinelectrolyte profileshepatic oxidative stressplasma liver biomarkersTridax procumbensACE: Public Health and Toxicological ResearchACE-PUTORUniversity of Port HarcourtNigeriaDamiete P. JackCaleb N. IkpeSamuel Ehiosun,Tosin B. AjayiArticle