Browsing by Author "Emokpae, Mathias Abiodun"

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    Alteration of neutral-alpha glucosidase in seminal plasma and correlation with sperm motility among men investigated for infertility Nigeria: a cross-sectional study
    (Fertility Science and Research, 2020-06-30) Moronkeji, Muyiwa Adeleye; Emokpae, Mathias Abiodun
    Introduction: Proteomic studies are becoming popular lately among reproductive biologists in the diagnosis and management of several diseases including male infertility. Objective: To evaluate the level of Neutral-alpha Glucosidase (NAG) in the seminal plasma of men being investigated for infertility in and to correlate its activity with sperm motility. Materials and Methods: Four hundred men age range 23–60 years were consecutively recruited in the study. After physical and clinical evaluation, the semen was collected by self or assisted masturbation and analysis was done according to the World Health Organization guidelines. The semen was then centrifuged and seminal plasma separated for the assay of NAG activity by ELISA technique using reagents supplied by Melson Medical Co, Shangai, China within one week of collection. The participants were categorized in to normozoospermia (191), oligozoospermia (98), severe oligozoospermia (70) and azoospermia (41) based on sperm count. Results: The mean levels of NAG activity decreased with decreasing levels of sperm count with values lowest among azoospermia and highest among normozoospermic subjects. The comparison of NAG activity between the various categories and control subjects was significantly different (P<0.001) except for normozoospermia which was not significantly different (P>0.05) when compared with controls. The mean NAG activity level was significantly lower among the asthenozoospermia (P<0.001) compared with normozoospermia. The NAG activity correlated positively with percentage sperm motility (r=0.126; P<0.02) and percentage sperm count (r=0.107; P<0.05). Conclusion: The findings indicate that NAG can be used for the assessment of semen quality as an adjunct to traditional semen analysis.
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    Alterations in Lipid Profile and Indices of Atherogenicity among Infertile Women Seeking Conception by Assisted Reproductive Technology
    (Jornal of Infertility Reproductive Biology, 2020-09-20) Emokpae, Mathias Abiodun; Eromosele, Claudius Osakhuemen; Ajibad, Hope Oluwadara
    Female infertility is common all over the world and lipid disorders are suggested to play a role. This study aimed to evaluate the plasma lipid profile and indices of atherogenicity among infertile women attending assisted reproductive technology clinics. Serum lipid profile (total cholesterol, triglycerides, low- density lipoprotein cholesterol, high- density lipoprotein cholesterol, and calculated indices of atherogenicity were determined in 140 infertile women and 50 healthy age-matched women of proven fertility. The lipid profile was assayed by the spectrophotometric method using reagents supplied by Randox Laboratories, Crumlin, Antrim, UK. The means values of measured parameters were compared between cases and controls by unpaired Students-test. The results showed that age (p<0.001), total cholesterol, triglycerides, low-density lipoprotein, AIP, some cardiac risk ratios, and atherogenic coefficients were significantly higher (p<0.001) while high-density cholesterol was significantly lower (p<0.001) among infertile women than control subjects. The difference in the mean body mass index between the cases and controls was not significant. The calculated indices of atherogenicity except high density/low-density ratio were significantly higher among infertile women seeking assisted reproductive technology for conception than control subjects. The higher indices of atherogenicity among this group of subjects might predispose them to cardiovascular diseases. Therefore, routine evaluation of lipid profile and indices of atherogenicity is suggested.
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    Seminal Plasma concentrations of Semenogelin and Zinc Among infertile males and their association with Asthenozoospermia.
    (BJMHR- British Journal of Medical and Health Research, 2020) Morokeji, Muyiwa Adeleye; Emokpae, Mathias Abiodun
    Poor semen liquefaction is one of the causes of male infertility which has become a public health challenge in Nigeria. Abnormal sperm motility is one of the commonest abnormalities detected in infertile males. The pathogenesis of poor liquefaction and solidification of semen are modulated by Semenogelin and some other secretions from male accessory organs. To evaluate the concentrations of Semenogelin and zinc in seminal plasma and to correlate the levels with percentage sperm motility. Four hundred men, mean age 36.4±6.8 years who were evaluated for infertility and 100 men, mean age 40.2±6.6 years of proven fertility were consecutively recruited for the study. Following routine semen analysis, seminal plasma Semenogelin-1 and zinc were determined by ELISA technique and Atomic Absorption spectrophotometry techniques respectively. Data generated were compared between groups and association between Semenogelin, zinc and percentage sperm motility was determined. Seminal plasma Semenogelin level was higher (p<0.001) in infertile males irrespective of the sperm concentration than controls. On the other hand, seminal plasma zinc level was lower (p<0.001) among oligozoospermia and azoospermia than controls. The mean Semenogelin level was significantly higher (p<0.001) among asthenozoospermia than those with normal percent motility while mean seminal plasma zinc level in asthenozoospermia was lower (p<0.001) than those with normal percentage motility. Seminal plasma Semenogelin (R=-0.217; p<0.005) correlated negatively with asthenozoospermia and zinc (R=-0.148;p<0.05) but zinc correlated (R-0.150;p<0.05) positively with asthenozoospermia. Alterations in the levels of Semenogelin and zinc are associated with asthenozoospermia among infertile males.