Immune Response in Mice Immunized with Chimeric H1 Antigens
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Date
2021-10-15
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
: Identification of a universal influenza vaccine candidate has remained a global challenge
for both humans and animals. This study describes an approach that uses consensus sequence
building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of
conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9
HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized
mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs
inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay.
These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas
of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds
potential for further development of a universal influenza vaccine.
Description
Keywords
universal influenza vaccine, broad-reactive antibodies, chimeric haemagglutinin, therapeutic; prophylactic, seasonal influenza, cross-reactivity, Jean-Remy Sadeyen, Munir Iqbal, Osbourne Quaye
Citation
https://doi.org/10.3390/vaccines9101182