Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana
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Date
2021-06-26
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Ethanolic extracts of samples of temperate zone propolis, four from the UK and one from
Poland, were tested against three Trypanosoma brucei strains and displayed EC50 values < 20 g/mL.
The extracts were fractionated, from which 12 compounds and one two-component mixture were
isolated, and characterized by NMR and high-resolution mass spectrometry, as 3-acetoxypinobanksin,
tectochrysin, kaempferol, pinocembrin, 40-methoxykaempferol, galangin, chrysin, apigenin, pinostrobin,
cinnamic acid, coumaric acid, cinnamyl ester/coumaric acid benzyl ester (mixture), 40,7-
dimethoxykaempferol, and naringenin 40,7-dimethyl ether. The isolated compounds were tested
against drug-sensitive and drug-resistant strains of T. brucei and Leishmania mexicana, with the highest
activities 15 M. The most active compounds against T. brucei were naringenin 40,7 dimethyl ether
and 40methoxy kaempferol with activity of 15–20 M against the three T. brucei strains. The most
active compounds against L. mexicana were 40,7-dimethoxykaempferol and the coumaric acid ester
mixture, with EC50 values of 12.9 3.7 M and 13.1 1.0 M. No loss of activity was found with
the diamidine- and arsenical-resistant or phenanthridine-resistant T. brucei strains, or the miltefosineresistant
L. mexicana strain; no clear structure activity relationship was observed for the isolated
compounds. Temperate propolis yields multiple compounds with anti-kinetoplastid activity.
Description
Journals of Molecules Vol 26 (3)
Keywords
temperate propolis, flavonoids, isolated compounds, Trypanosoma brucei, Leishmania mexicana, Ibrahim A. Alfayez, Manal J. Natto, Sameah Alenezi, Weam Siheri, Malik AlQarni, John O. Igoli, James Fearnley, Harry P. De Koning, David G. Watson, Benue State University, Nigeria, CEFTER
Citation
Alotaibi, A., Ebiloma, G. U., Williams, R., Alfayez, I. A., Natto, M. J., Alenezi, S., ... & Watson, D. G. (2021). Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana. Molecules, 26(13), 3912.